This archive contains digital microscopic images of HeLa cells
incubated with styryl compound G52, along with other information
about the compound.  It is part of a larger collection of archives for
1344 styryl compounds.

The compounds were synthesized in a combinatorial synthesis involving
8 pyridinium/quinolinium groups (A-H) and 168 aldehyde groups (1-168).
The compounds are identified by their A-H letter followed by their
1-168 number.

The files in this tar archive are as follows:

Hoechst_withdye.bz2         Hoechst channel 50msec exposure "withdye" image.
FITC1s_withdye.bz2          FITC channel 1sec exposure "withdye" image.
TRITC1s_withdye.bz2         TRITC channel 1sec exposure "withdye" image.
Cy51s_withdye.bz2           Cy5 channel 1sec exposure "withdye" image.
FITC200ms_withdye.bz2       FITC channel 1sec exposure "withdye" image.
TRITC200ms_withdye.bz2      TRITC channel 1sec exposure "withdye" image.
Hoechst_washout.bz2         Hoechst channel 50msec exposure "washout" image.
FITC1s_washout.bz2          FITC channel 200msec exposure "washout" image.
TRITC1s_washout.bz2         TRITC channel 200msec exposure "washout" image.
Cy51s_washout.bz2           Cy5 channel 200msec exposure "washout" image.
FITC200ms_washout.bz2       FITC channel 200msec exposure "washout" image.
TRITC200ms_washout.bz2      TRITC channel 200msec exposure "washout" image.
G52.sdf.bz2                 The chemical structure of G52 in SDF format.
G52.png                     A depiction of the 2D structure of G52.


The archive is in standard tar format and can be extracted using GNU
tar or other software conforming to the tar standard.  The files
within the tar archive (except for the png image) are bzip2
compressed.

Each of the 12 images in this archive is a 512x512 pixel image stored
as a bzip2 compressed sequence of 2 byte unsigned short integers
(little endian format), in standard raster order.

Fluorescence in the Hoechst channel derives from Hoechst dye.
Fluorescence in the other channels is presumed to derive from the
styryl molecules, although cellular autofluorescence is also present.

The images were acquired at 200X magnification with a 20X objective.
The images were acquired with a 12 bit CCD camera so the greyscale
intensities range from 0 to 4095.

The "withdye" images were acquired after around 1 hour of incubation
with the styryl probe.  Next the probe was washed out using an onboard
robotic pipetting system, and the "washout" images were acquired.

The Hoechst dye images were acquired at 50msec exposure time, the other
channels were acquired at both a 1sec and 200msec exposure time (Cy5
was only acquired at 1sec).

The images were obtained using a Cellomics KineticScan instrument.
Cells were kept alive and healthy in an onboard environmental control
chamber.

Styryl compounds were present at 100 micromolar concentration.

